Key features and details | |
Cat. No. | MABL-1882 |
Name | Anti-HPr mAbs |
Clone No. | AFD- JEL42 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA |
Species Reactivity | Escherichia coli |
Basic Information | |
Specificity | This antibody binds the histidine containing phosphocarrier protein of Escherichia coli. It is an 85 amino acid phosphocarrier protein which is component of phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. |
Alternative Name | Histidine-containing phosphocarrier protein; ptsH; hpr; Phosphocarrier protein HPr; Histidine-containing protein |
UniProt | P0AA04 |
Immunogen | |
Application Notes | This antibody can detect mutant histidine binding protein in crude extracts. It was also capable of inhibiting PTS sugar phosphorylation assays, but the antibody-HPr complex could still be phosphorylated by enzyme I (PMID: 2438269) |
Antibody First Published | Waygood et al. Characterization of mutant histidine-containing proteins of the phosphoenolpyruvate: sugar phosphotransferase system of Escherichia coli and Salmonella typhimurium. J Bacteriol. 1987 Jun; 169(6): 2810–2818. PMID:2438269 |
Note on publication | Describes the characterization of mutant HPr protein of E. coli using two different antibodies. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
