Anti-HSP65 mAbs

Cat. No.

MABL-1885

Name

Anti-HSP65 mAbs

Clone No.

AFD- G8

From

Recombinant Antibody

Isotype

Engineer antibody

Application

WB, ELISA

Species Reactivity

Mycobaterium bovis

Basic Information

Specificity

This antibody is specific for the HSP65 protein from Mycobacterium bovis. Together with its co- chaperonin GroES,   HSP65 plays an essential role in assisting protein folding. Mycobacterium bovis is the causative agent of tuberculosis in cattle.

Alternative Name

Chaperonin-60 2; Heat shock protein 65; Chaperonin GroEL 2; 60 kDa chaperonin 2; 65 kDa antigen; Antigen A; Cell wall protein A

UniProt

P0A521

Immunogen

This antibody was generated by panning a phage displayed repertoire derived from the immunoglobulin genes of the chicken against HSP65.

Application Notes

An ELISA was performed using the scFv   version of this antibody on HSP65 to determine how efficiently the antibody   can bind the antigen. Furthermore, an immunoblot was performed on HSP65 using   this antibody (Wemmer et al, 2010; pmid:20299243).

Antibody First   Published

Wemmer et al. Chicken scFvs and bivalent   scFv-C(H) fusions directed against HSP65 of Mycobacterium bovis. Biologicals.   2010 May;38(3):407-14. PMID:20299243

Note on publication

Two chicken single-chain variable region  antibody fragments (scFvs) that recognised the 65 kDa heat-shock protein (HSP65) of Mycobacterium bovis were selected from a large semi-synthetic phage displayed library. Both recognised HSP65 in indirect enzyme-linked   immunosorbent assay (ELISA) and immunoblots and retained their activity   during storage.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.