Anti-HSP70 mAbs

Cat. No.

MABL-1886

Name

Anti-HSP70 mAbs

Clone No.

AFD- cmhsp70.1 (C92F3B1)

From

Recombinant Antibody

Isotype

Engineer antibody

Application

WB, ELISA, FC, IF, IHC

Species Reactivity

Human

Basic Information

Specificity

This antibody binds a region including amino   acids 473-504 (TKDNNLLGRFELSG) of the C- terminal substrate-binding domain   (SBD) of membrane bound Hsp70 (mHsp70). It binds a 14-mer peptide termed”.   This protein is located intracellularly and plays an important part in   various cellular processes, including protection of the proteome from stress,   folding and transport of newly synthesized polypeptides, activation of   proteolysis of misfolded proteins and the formation and dissociation of   protein complexes. It plays a pivotal role in the protein quality control   system, ensuring the correct folding of proteins, the re- folding of   misfolded proteins and controlling the targeting of proteins for subsequent   degradation.

Alternative Name

HSP70-1; HSP70.1; HSPA1A; HSX70; HSP72;   mHsp70; Heat shock 70 kDa protein 1A; Heat shock 70 kDa protein 1

UniProt

P0DMV8

Immunogen

The original antibody was generated by   grafting the CDRs of the mouse antibody on human framework regions. The parental   mouse antibody was generated by immunizing mice with an SBD-derived synthetic   peptide TKDNNLLGRFELSG (TKD peptide; aa 450–463) with complete Freud’s   adjuvants.

Application Notes

The IgG1 version of this antibody binds the   C-terminal substrate-binding domain (SBD) of the human membrane heat shock   protein mHsp70 with an affinity of 3nM. The binding characterization of this   antibody was done using ELISA. This antibody can detect aa 473–504) fused to   domain III of HSA in a western blot assay (PMID: 20556545). The Fab version  of this antibody binds mHsp70 with an affinity of 35 nM. The fab version can   successfully identify membrane-associated Hsp70 on tumor cell lines both in   immunofluorescence staining and flow cytometry (PMID: 20123884). The original   mouse antibody cmHsp70.1 can selectively induce antibody-dependent cellular   cytotoxicity (ADCC) of membrane Hsp70+ mouse tumor cells by unstimulated   mouse spleen cells. 

Antibody First   Published

Zettlitz et al. Humanization of a mouse   monoclonal antibody directed against a cell surface-exposed epitope of   membrane-associated heat shock protein 70 (Hsp70). Mol Biotechnol. 2010   Nov;46(3):265-78. PMID:20556545

Note on publication

Describes the humanization of a mouse   antibody against membrane-associated heat shock protein 70 (Hsp70).

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.