Anti-HTT-N17 mAbs

Cat. No.

MABL-1894

Name

Anti-HTT-N17 mAbs

Clone No.

AFD- scFv-C4

From

Recombinant Antibody

Isotype

Engineer antibody

Application

crystallization, in vitro, SPR, ELISA, IF

Species Reactivity

Human

Basic Information

Specificity

The antibody is specific for huntingtin. It   binds to the first 17 residues of huntingtin [HTT(1- 17)].

Alternative Name

C4; HTT; Huntingtin; HD protein; Huntington   disease protein

UniProt

P42858

Immunogen

The original antibody was isolated screening   a naïve human single-chain Fv antibody phage- display library against   HTT(1-17).

Application Notes

The original format of the antibody could   inhibit the aggregation of mHTT-exon1 protein fragments in vitro. The crystal   structure of the antibody in complex with HTT(1-17) was determined (De Genst   et al., 2015; PMID: 25861763). The specificity of the antibody was confirmed by ELISA analysis. The original format of the antibody-targeted the   HD-Q103-myc antigen to cell nuclei in COS-7 cells by immunofluorescence   microscopy. The binding affinity of the antibody to HTT(1-17) was measured by   surface plasmon resonance (Kd= 7.9 nM) (Lecerf et al., 2001; PMID: 11296304).   The antibody selectively bound diffuse htt, but not aggregated htt, as shown   by immunofluorescence analysis. Immunoprecipitation experiments in stably   transfected, intrabody-expressing STHdhQ111 cells demonstrated that the   antibody bound a small fraction of wild-type and mutant endogenous   full-length htt (Miller et al., 2005; PMID: 15837560).

Antibody First   Published

Lecerf et al., Human single-chain Fv   intrabodies counteract in situ huntingtin aggregation in cellular models of   Huntington's disease. Proc Natl Acad Sci U S A. 2001 Apr 10;98(8):4764-9.   doi: 10.1073/pnas.071058398. PMID:11296304

Note on publication

The original paper describes the generation   and characterization of the antibody.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.