Anti-HTT mAbs

Cat. No.

MABL-1893

Name

Anti-HTT mAbs

Clone No.

AFD- iVHH4

From

Recombinant Antibody

Isotype

Engineer antibody

Application

IP, WB, ELISA

Species Reactivity

Human

Basic Information

Specificity

The antibody is specific for wild-type and   mutant human huntingtin under native and denatured conditions. The antibody  epitope is located between amino acids 49–148.

Alternative Name

HTT; Huntingtin; HD protein; Huntington   disease protein

UniProt

P42858

Immunogen

The original antibody was generated by immunizing   a llama with N-terminal htt protein, followed by phage-VHH display library construction.The antibody was isolated by biopanning against wild- type or   mutant N-terminal htt fragment.

Application Notes

The specificity of the original format of the antibody was confirmed by ELISA analysis. The antibody detected the htt   by western blot analysis. The antibody could co-immunoprecipitate htt from   human HD brain lysates (Schut et al., 2015; PMID: 25294428).

Antibody First   Published

Schut et al. Selection and characterization   of llama single domain antibodies against N-terminal huntingtin Neurol Sci.   2015 Mar;36(3):429-34. doi: 10.1007/s10072-014-1971-6. Epub 2014 Oct 8.   PMID:25294428

Note on publication

The original paper describes the generation and characterization of the antibody.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.