Key features and details | |
Cat. No. | MABL-1904 |
Name | Anti-Human IgG mAbs |
Clone No. | AFD- 17F12 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA |
Species Reactivity | rhesus macaque, Human, Cynomolgus Monkey |
Basic Information | |
Specificity | This antibody binds to all four human IgG subtypes (IgG1, IgG2, IgG3 & IgG4). Some cross- reactivity with rabbit IgG, cynomolgus monkey IgG1 and rhesus macaque IgG1 has also been reported. |
Alternative Name | Immunoglobulin G |
UniProt | |
Immunogen | This antibody was raised against human IgG. |
Application Notes | A Fab and an scFv fragment derived from this antibody, conjugated with bacterial alkaline phosphatase, have been used in sensitive enzyme immunoassays for the detection of human IgG (Carrier et al, 1995). Further, the antibody reacted with human IgG1, cynomolgus monkey IgG1 and rhesus macaque IgG1 by ELISA analysis, but it did not show any cross reactivity with cynomolgus monkey IgG4. |
Antibody First Published | Carrier et al. Recombinant antibody-alkaline phosphatase conjugates for diagnosis of human IgGs: application to anti-HBsAg detection Journal of Immunological Methods, 1995, Vol.181(2), pp.177-186 PMID:7745247 |
Note on publication | Describes the derivation of an scFv and Fab from this antibody, and their use in a sandwich ELISA. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
