Key features and details | |
Cat. No. | MABL-1905 |
Name | Anti-Human IgG mAbs |
Clone No. | AFD-8E 11 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA, IF |
Species Reactivity | chimpanzee, mangabey, vervet, Human |
Basic Information | |
Specificity | This antibody reacts with human IgG1, IgG2, IgG3, and IgG4, as well as mangabey, vervet and chimpanzee IgG. It has minimal reactivity with rhesus macaque, cynomolgus monkey or pigtailed macaque IgG. |
Alternative Name | Human immunoglobulin G |
UniProt | |
Immunogen | This antibody was raised by immunising mice with baboon IgG and screening hybridomas against IgG of different primate species. |
Application Notes | The ability of this antibody to distinguish between human and macaque IgG has been confirmed in ELISA analysis. As a result of this specificity, this antibody has been used in immunofluorescence analysis to detect intravenously-administered human IgG in formaldehyde-fixed rhesus tissue sections (Schneider et al, 2017). |
Antibody First Published | Schneider et al. Long-term direct visualization of passively transferred fluorophore-conjugated antibodies. J Immunol Methods. 2017 Nov;450:66-72 PMID:28780040 |
Note on publication | This antibody is from the laboratory of Keith Reimann, DVM, Beth Israel Deaconess Medical Center. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
