Key features and details | |
Cat. No. | MABL-2036 |
Name | Anti-IL-17A mAbs |
Clone No. | AFD- 1G8 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | WB, ELISA, FC |
Species Reactivity | Chicken |
Basic Information | |
Specificity | The antibody binds specifically to chicken IL-17, a proinflammatory cytokine produced by activated T cells. Further, The antibody binds to duck IL-17. |
Alternative Name | Interleukin 17A; chIL-17; Interleukin-17A; IL-17; chicken IL-17A |
UniProt | B4ER12 |
Immunogen | The original antibody was raised by immunizing BALB/C mice with recombinant chicken IL-17. |
Application Notes | The specificity of this antibody (IgG1) was confirmed in ELISA analysis. The antibody detected the recombinant chIL-17A by western blot. This antibody was used for flow cytometry on CU205 cells (Yoo et al, 2008; pmid: 18022248). The antibody was used to study the interactions between chIL-17RA and chIL-17A by western blot (Kim et al, 2014; pmid: 24980970). The cross-reactivity of the antibody to chIL17 with duck IL17 was evaluated by indirect ELISA. The binding intensity of the antibody to recombinant chIL17 was similar to recombinant duIL17. The antibody detected duIL-17A by western blot (Yoo et al., 2009; pmid: 19573930). |
Antibody First Published | Yoo et al. Monoclonal antibodies reactive with chicken interleukin‐17. Vet Immunol Immunopathol. 2008;121(3‐4):359 ‐363. PMID:18022248 |
Note on publication | The article describes the generation and characterization of the antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
