Key features and details | |
Cat. No. | MABL-2051 |
Name | Anti-IL-1B mAbs |
Clone No. | AFD-1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IH, neutralizing, WB, ELISA |
Species Reactivity | Chicken |
Basic Information | |
Specificity | The antibody is specific for chicken IL-1β, a pleiotropic molecule with a wide spectrum of activities including metabolic, physiologic, hematologic, and immunologic effects. The antibody did not react with chicken IL-18. |
Alternative Name | Interleukin IL1‐beta; chIL-1B-1; Interleukin-1; chIL-1B |
UniProt | O73909 |
Immunogen | The original antibody was raised immunising BALB/C mice with recombinant chicken IL-1B protein. |
Application Notes | The specificity of the original format of this antibody was confirmed by ELISA analysis (Lee et al, 2014; pmid: 25037821). The antibody detected chIL-1β protein by Western blot analysis. The antibody was used as a capture antibody for measuring serum IL-1B levels in chicken by capture ELISA analysis. The original format of the antibody was used to localize IL-1β-secreting cells in chicken immune tissues. The antibody neutralized the bioactivity of chIL-1B-induced cell proliferation and NO production by primary thymocytes (Lee et al, 2014; pmid: 25037821). |
Antibody First Published | Lee et al. Development and characterization of mouse monoclonal antibodies reactive with chicken IL-1β Poult Sci. 2014;93(9):2193‐2198. PMID:25037821 |
Note on publication | The paper describes the generation and characterization of the antibody, which is specific for chIL-1β. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
