Key features and details | |
Cat. No. | MABL-2052 |
Name | Anti-IL-1B mAbs |
Clone No. | AFD- 1B12.4H4 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | functional assay, ELISA |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody binds human Interleukin-1 beta, which is a potent pro-inflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. It promotes Th17 differentiation of T-cells and synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. It also plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6. |
Alternative Name | IL1B; IL-1β; 1L1F2; Interleukin-1 beta; IL-1 beta; Catabolin |
UniProt | P01584 |
Immunogen | The original antibody was generated by immunizing mice with recombinant human IL-1 beta. |
Application Notes | The original mouse IgG antibody binds human IL-1B with a binding affinity of Kd= 0.26 nM. The binding characterization of this antibody was done using ELISA. In vitro antibody potency was determined by its ability to inhibit IL-8 production by MRC-5 cells. This antibody was able to inhibit IL-8 production by MRC-5 cells with an IC50 of 5.30E−10M. This antibody was used in combination with 18F4.2C8 (anti-IL-1α) antibody for the generation of anti-human IL-1α/β DVD-Ig molecule that had therapeutic potential (PMID: 20068402). |
Antibody First Published | Wu et al. Molecular construction and optimization of anti-human IL-1α/β dual variable domain immunoglobulin (DVD-Ig™) molecules. MAbs. 2009 Jul-Aug; 1(4): 339–347. PMID:20068402 |
Note on publication | Describes the generation of anti-human IL-1α/β DVD-Ig™ molecules using several pairs of monoclonal antibodies with therapeutic potential. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
