Key features and details | |
Cat. No. | MABL-2114 |
Name | Anti-IL-8RA mAbs |
Clone No. | AFD- SE2 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | WB, FC, IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | SE2 antibody is specific for IL-8RA and does not corss-react with IL-8RB. IL-8RA is a receptor to interleukin-8, which is a powerful neutrophils chemotactic factor. Binding of IL-8 to the receptor causes activation of neutrophils. |
Alternative Name | CXCR-1; C-X-C chemokine receptor type 1; CXC-R1; CDw128a; High affinity interleukin-8 receptor A; IL-8R A; IL-8 receptor type 1; CD181; interleukin 8 |
UniProt | P25024 |
Immunogen | This antibody was raised by immunizing BALB/c mice with a BSA-conjugated peptide comprising of the N-terminal part of IL-8RA (amino acids 1-30). |
Application Notes | This antibody is recommended for the detection and analysis of IL-8RA expression. One group used it to identify IL-8RA on the surface of human granulocytes via FACS (Zahn et al., 1997). Importantly, SE2 antibody was specific to IL-8RA-expressing cells only. Subsequent flow cytometric analysis with this antibody led to the characterization of IL-8RA density on human leukocytes (Zahn et al., 1997). |
Antibody First Published | Zahn et al. Chemoattractant receptors for interleukin-8 and C5a: expression on peripheral blood leukocytes and differential regulation on HL-60 and AML-193 cells by vitamin D3 and all- trans retinoic acid. Eur J Immunol. 1997 Apr;27(4):935-40. PMID:16144840 |
Note on publication | This article describes the generation and characterization of SE2 antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
