Key features and details | |
Cat. No. | MABL-2132 |
Name | Anti-inactivated vaccinia virus mAbs |
Clone No. | AFD- VACC-E7 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA |
Species Reactivity | Vaccinia virus |
Basic Information | |
Specificity | The antibody is specific for vaccinia virus. The antibody does not recognize the L1 antigen. Vaccinia virus is a large, complex, enveloped virus belonging to the poxvirus family. |
Alternative Name | killed vaccinia virus; VV; VACV |
UniProt | |
Immunogen | Two llamas were immunized with a combination of killed vaccinia virus material and purified recombinant L1 antigen. A VHH library was then constructed and panned against killed vaccinia virus . |
Application Notes | The specificity of the original format of the antibody was confirmed by ELISA analysis. The antibody was assayed as both capture and tracer in MAGPIX bead-based sandwich assays for the detection of vaccinia virus and a limit of detection (LOD) of 4x10^5 pfu/ml was achieved (Walper et al., 2014; PMID: 25211488). |
Antibody First Published | Walper et al. Development and evaluation of single domain antibodies for vaccinia and the L1 antigen PLoS One. 2014 Sep 11;9(9):e106263. PMID:25211488 |
Note on publication | The original paper describes the generation and characterization of the antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
