Anti-Indolicidin mAbs

Cat. No.

MABL-2133

Name

Anti-Indolicidin mAbs

Clone No.

AFD- V2D2

From

Recombinant Antibody

Isotype

Engineer antibody

Application

Block, ELISA

Species Reactivity

Cow

Basic Information

Specificity

This antibody binds indolicidin   (ILPWKWPWWPWRR), which is a potent antimicrobial peptide isolated from the   neutrophil blood cells of cow. Indolicidin is a tryptophan-rich cationic   broad spectrum antibiotic that can neutralize bacterial lipopolysaccharides   and has immunomodulatory effects. Its antibacterial activity is realized   inside the phagolysosomes of host neutrophils, outside of which it exhibits   cytotoxicity. Some reports have demonstrated its interaction with various   molecules like DNA, topoisomerase I, calmodulin, and ATP.

Alternative Name

CATHL4; Cathelicidin-4; Antimicrobial peptide; antibiotic

UniProt

P33046

Immunogen

The original antibody was generated by immunizing   8–10-week-old BALB/c mice with indolicidin conjugated to diphtheria toxoid.

Application Notes

The binding characterization of this   antibody was done using ELISA. It was reported that this antibody could bind   indolicidin with a binding affinity of KD = 56 nM and could block the   antibacterial action of the peptide (PMID: 20837490).

Antibody First   Published

Lomash et al. An antibody as surrogate receptor reveals determinants of activity of an innate immune peptide   antibiotic. J Biol Chem. 2010 Nov 12;285(46):35750-8. PMID:20837490

Note on publication

Describes the generation of this antibody as   a surrogate receptor for indolicidin to identify a motif that contained   almost the entire antibiotic activity of native indolicidin.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.