Anti-Integrin alpha-L mAbs

Cat. No.

MABL-2156

Name

Anti-Integrin alpha-L mAbs

Clone No.

AFD- D2-57

From

Recombinant Antibody

Isotype

Engineer antibody

Application

crystallography, in vitro, SPR, ELISA, FC, IF

Species Reactivity

Human

Basic Information

Specificity

This antibody is specific for the LFA-1 I   domain in a HA conformation.

Alternative Name

AL-57; CD11a; ITGAL; CD11 antigen-like   family member A; Leukocyte adhesion glycoprotein LFA-1 alpha chain; Leukocyte   function-associated molecule 1 alpha chain

UniProt

P20701

Immunogen

The original antibody was raised using human   phage display library FAB-300. To screen the library purified locked-open HA,   locked-closed LA, and wild-type I domains of LFA-1 were used.

Application Notes

The original antibody was used for an ELISA   on LFA-1 HA I domain. This antibody was used for immunofluorescence on K562   cells with different LFA-1 conformations. Furthermore this antibody was used   for flow cytometry on K562 cells with different LFA-1 conformations (Huang et   al., 2006; PMID:16888085), and on IL-15-cultured T lymphocytes (Shimaoka et   al., 2006; PMID:16963559). This antibody was used for an ICAM-1 blocking   assay on HA cells, to determine the effect of the antibody to inhibit ligand   binding. The antibody was used for a PHA-induced lymphocyte proliferation   assay on PBMCs, to determine the effect of the antibody on these cells. It   did indeed block PHA-induced lymphocyte proliferation (Huang et al., 2006;   PMID:16888085). 

Antibody First   Published

Huang et al. Identification and   characterization of a human monoclonal antagonistic antibody AL-57 that   preferentially binds the high-affinity form of lymphocyte function-   associated antigen-1 J Leukoc Biol. 2006 Oct;80(4):905-14. PMID:16888085

Note on publication

The original paper reports the   identification and characterization of a human antibody AL-57, which binds to   the alpha(L) I domain in a HA but not LA conformation.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.