Key features and details | |
Cat. No. | MABL-2157 |
Name | Anti-Integrin alpha.sub.v.beta.sub.3 mAbs |
Clone No. | AFD- LM609 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | crystallography, inhibition, IP, therapheutic, FC, IF, IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | The antibody specifically recognizes αVβ3 integrin. In particular, it recognizes an epitope on the αVβ3 integrin headpiece apex at the interface between the β-propeller and βI domains, sterically hindering access of large ligands to the RGD binding pocket. |
Alternative Name | αvβ3; αVβ3 integrin; alphav beta3 integrin; Vitronectin receptor; Vitronectin receptor subunit alpha; CD51; MED-523; Vitaxin; Etaracizumab; Abegrin |
UniProt | P06756 |
Immunogen | The original antibody was generated by immunizing BALB/c mice with purified cell adhesion receptor. |
Application Notes | The antibody inhibited the attachment of human endothelial cells to fibrinogen, von Willebrand factor, and vitronectin (Cheresh et al, 1987; pmid:2442758). The antibody was used in FACS analysis on M21 cells. The antibody was used for immunoprecipitation analysis on M21 cells. The antibody is capable of preventing M21 cell attachment to vitronectin, von Willebrand factor, fibrinogen, and the Arg-Gly- Asp peptide (Cheresh et al, 1987; pmid:2447074). Flow cytometry analysis of integrin ανβ3 expression profiles of HUVEC was performed using this antibody. The antibody was used immunofluorescence against integrin ανβ3 (Diaz et al, 2020; pmid:31780581). The antibody was used in immunohistochemistry (Mena et al, 2014; pmid:24973039). The antibody blocked the binding of fibronectin and vitronectin proteins to ανβ3 in a solid-phase microtiter assay. In vitro studies demonstrated the antibody inhibits fibronectin binding to αVβ3 (Charo et al., 1990; pmid:1703545). |
Antibody First Published | Cheresh et al. Human endothelial cells synthesize and express an Arg-Gly- Asp-directed adhesion receptor involved in attachment to fibrinogen and von Willebrand factor. Proc Natl Acad Sci U S A. 1987 Sep;84(18):6471-5. PMID:2442758 |
Note on publication | The paper describes the generation and characterization of the antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
