Key features and details | |
Cat. No. | MABL-2159 |
Name | Anti-Integrin beta-7 mAbs |
Clone No. | AFD- FIB27 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, Block, FC |
Species Reactivity | Human, Mouse |
Basic Information | |
Specificity | FIB27 binds specifically to both human and mouse integrin beta-7 at epitope region Dl on which is involved in all a4b7-mediated adhesion events. Does not compete with DAKT32 for binding. FIB27 blocks LS722-induced TK1 cell aggregation (mAb LS722 is an activating anti-a4B7 antibody and induces aggregation via an a4B7-dependent pathway). Integrin beta-7 is one subunit is a heterodimer which makes up an intergin molecule. In the mouse beta-7 is selectively found on the majority of mature lymphocytes, whereas a small subpopulation of thymocytes and bone marrow cells express beta-7. Beta-7 is also expressed in variable amounts in CD4 T memory cells. |
Alternative Name | FIB-27; FIB 27; Fib-27; Fib27; Fib 27; fib27; fib-27; fib 27; Integrin b7; integrin b-7; ITGB7; Gut homing receptor beta subunit |
UniProt | P26010 (human); P26011 (mice) |
Immunogen | FIB27 was prepared by immunizing rats with TK1 cells. |
Application Notes | FIB27 can be used for FC, IP and can block TKI cell aggregation and beta-7 intergrin- mediated cell adhesion. |
Antibody First Published | Andrew DP, et al. Distinct but overlapping epitopes are involved in alpha 4 beta 7-mediated adhesion to vascular cell adhesion molecule-1, mucosal addressin-1, fibronectin, and lymphocyte aggregation. J. Immunol. 153:3847 1994 PMID:7523506 |
Note on publication | Describes the use of FIB27 in defining functional binding regions of alpha-4/beta-7 integrin by epitope mapping. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
