Key features and details | |
Cat. No. | MABL-2162 |
Name | Anti-integrin α5β1 mAbs |
Clone No. | AFD- 7H5 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | functional assay, ELISA, FC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody is specific for human integrin α5β1. |
Alternative Name | Integrin alpha-5; ITGA5; CD49 antigen-like family member E; Fibronectin receptor subunit alpha; Integrin alpha-F; VLA-5; CD49e |
UniProt | P08648 |
Immunogen | The original mouse version of this antibody was raised by immunizing mice with purified human alpha5beta1. Integrin alpha-5/beta-1 (ITGA5:ITGB1) is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. ITGA5:ITGB1 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1. ITGA5:ITGB1 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D- dependent cell adhesion to FBN1. |
Application Notes | The murine version of this antibody was used in a HUVEC direct binding assay. In this assay the ability of the antibody to bind to HUVACs was tested (US2012195908). Furthermore, the murine version of this antibody was also utilized in flow ctyometry to stain RAJI cells and HUVEC cells. It only bound HUVECs and not RAJI cells (US2012195908). Additionally, it was determined that the murine version of this antibody could inhibit cell adhesion to fibronectin (US2012195908). An HUVEC Caspase-3/7 Activity Colometric Assay was preformed using the murine version of this antibod to determine the apoptosis resulting from the administration of the antibody (US2012195908). HUVEC tube formation assays were preformed using the murine version of this antibody (US2012195908). |
Antibody First Published | Wei-Ching Novel antibodies US20120148572A1 |
Note on publication | Describes the generation and characterization of this antibdy. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
