Anti-IRTA-5 mAbs

Cat. No.

MABL-2172

Name

Anti-IRTA-5 mAbs

Clone No.

AFD- 2G5

From

Recombinant Antibody

Isotype

Engineer antibody

Application

SPR, FC

Species Reactivity

Human

Basic Information

Specificity

2G5 is specific for IRTA5; it does not bind   IRTA1, IRTA2, IRTA3, or IRTA4.

Alternative Name

CD307a; Immune Receptor   Translocation-Associated Protein 5; Fc Receptor Like 1; FCRL1; FcR-like   protein 1; Fc receptor homolog 1; FcRH1

UniProt

Q96LA6

Immunogen

Mice of the HCo7 HuMab Mouse(R) strain were   immunized with purified recombinant IRTA5 fusion protein. Mouse splenocytes,   isolated from the HuMab mice, were fused with PEG to a mouse myeloma cell   line. The resulting hybridomas were then screened for the production of   antigen-specific antibodies. The 2G5 clone was selected from this.

Application Notes

SPR was used to determine the Kd of 2G5   binding to IRTA5 as 0.028 x 10^-9 M. Using flow cytometry 2G5 was shown to be   specific for IRTA5; it does not bind IRTA1, IRTA2, IRTA3, or IRTA4. Flow   cytometry also showed that 2G5 directed against human IRTA-5 binds to CD 19+   B cells and the B-cell tumor lines Karpas 1106P, SU-DHL-4, Granta 519, and L-540. 2G5 does not bind CD3+ peripheral blood T cells, CD 1A+ peripheral   blood dendritic cells, CD14+ peripheral blood monocytes, or CD56+ peripheral   blood NK cells.

Antibody First   Published

Note on publication

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.