Key features and details | |
Cat. No. | MABL-2198 |
Name | Anti-Keratin (4,5,6,8,10,13,18) mAbs |
Clone No. | AFD- C11 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | WB, IF, IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody recognizes the human keratin, types 4, 5, 6, 8, 10, 13, and 18. Because of its wide range in keratin specificity, it often is referred to in literature as pan-keratin. |
Alternative Name | Pan-keratin; Keratin, type II cytoskeletal 5; 58 kDa cytokeratin; Cytokeratin-5; Type-II keratin Kb5 |
UniProt | P13647 |
Immunogen | The original mouse IgG1 version of this antibody was raised by immunizing mice against human cytoskeleton |
Application Notes | This original IgG1 antibody can be used to detect human keratin. Its specificity has been confirmed using immunofluorescence (Fehm et al., 2002; PMID: 12114406). One group used immunofluorescence to test if circulating epithelial cells were malignant (Fehm et al., 2002; PMID: 12114406), which was later built upon with the discovery that these cells remained in circulation until many years after treatment (Meng et al., 2004; PMID: 15623589). Another group used the antibodies with immunofluorescence and immunohistochemistry to visualize the progression of breast cancer once it had breached the epithelial cell layer (Khalil et al., 2017; PMID: 28894989). A final group used this antibody with immunofluorescence and western blotting to research the apoptotic response of keracinocytes to ultraviolet-B light (Mei, Lian, 2011; PMID: 21362287). |
Antibody First Published | Kovarík et al. Monoclonal antibodies against individual cytokeratins in the detection of metastatic spread Int J Cancer Suppl. 1988;3:50-5 PMID:2463228 |
Note on publication | Is the first mention of the antibody by the same lab that raised it, and describes its usuage |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
