Key features and details | |
Cat. No. | MABL-2223 |
Name | Anti-LAG-3 mAbs |
Clone No. | AFD- G8 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | BLI, Blocking, inhibition, ELISA, FC |
Species Reactivity | rhesus macaque, Human |
Basic Information | |
Specificity | The antibody binds to human and rhesus macaque LAG-3. |
Alternative Name | Lymphocyte activation gene 3 protein; CD223 |
UniProt | P18627 |
Immunogen | The original antibody was isolated from a human antibody phage display library by bio- panning against human LAG-3. |
Application Notes | The specificity of the original format of the antibody was confirmed by ELISA analysis. The antibody was able to bind LAG-3 expressed at the surface of HEK-293 cells was evaluated by flow cytometry. The antibody showed binding to activated T cells. The antibody bound to cells expressing rhesus LAG-3 on transiently transfected HEK-293 cells. The affinity of the antibody to human LAG-3 was measured using Bio-Layer Interferometry (Kd = 0.70 nM). The antibody showed inhibitory capacity of the binding of LAG-3 to MHC class II in a dose dependent manner, and was able to completely block binding of LAG-3 to MHC-II at high concentrations (IC50 = 12 nM). The antibody could block the binding of LAG-3 to MHC class II- expressing Daudi cells in a dose-dependent manner by flow cytometry analysis. The antibody was able reverse exhaustion and restore the activity of T cells to secrete IL-2 and IFN-γ upon restimulation at high doses (US20190040136A1). |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
