Anti-LAG-3 mAbs

Cat. No.

MABL-2223

Name

Anti-LAG-3 mAbs

Clone No.

AFD- G8

From

Recombinant Antibody

Isotype

Engineer antibody

Application

BLI, Blocking, inhibition, ELISA, FC

Species Reactivity

rhesus macaque, Human

Basic Information

Specificity

The antibody binds to human and rhesus   macaque LAG-3.

Alternative Name

Lymphocyte activation gene 3 protein; CD223

UniProt

P18627

Immunogen

The original antibody was isolated from a   human antibody phage display library by bio- panning against human LAG-3.

Application Notes

The specificity of the original format of   the antibody was confirmed by ELISA analysis. The antibody was able to bind   LAG-3 expressed at the surface of HEK-293 cells was evaluated by flow   cytometry. The antibody showed binding to activated T cells. The antibody   bound to cells expressing rhesus LAG-3 on transiently transfected HEK-293   cells. The affinity of the antibody to human LAG-3 was measured using   Bio-Layer Interferometry (Kd = 0.70 nM). The antibody showed inhibitory   capacity of the binding of LAG-3 to MHC class II in a dose dependent manner,   and was able to completely block binding of LAG-3 to MHC-II at high   concentrations (IC50 = 12 nM). The antibody could block the binding of LAG-3   to MHC class II- expressing Daudi cells in a dose-dependent manner by flow   cytometry analysis. The antibody was able reverse exhaustion and restore the   activity of T cells to secrete IL-2 and IFN-γ upon restimulation at high   doses (US20190040136A1).

Antibody First   Published

Note on publication

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.