Key features and details | |
Cat. No. | MABL-2234 |
Name | Anti-Laminin mAbs |
Clone No. | AFD- A5 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, IF, IHC |
Species Reactivity | Bovine, Human, Mouse |
Basic Information | |
Specificity | This antibody recognizes and precipitates laminin, a macromolecule prevalent in the basement membrane responsible for the attachment of cells to each other and forming tissue in the embryonic development of an organism. |
Alternative Name | S-LAM gamma; Laminin subunit gamma-1; Laminin B2 chain; Laminin-1 subunit gamma; Laminin-2 subunit gamma; Laminin-3 subunit gamma; Laminin-4 subunit gamma; Laminin- 6 subunit gamma; Laminin-7 subunit gamma; Laminin-8 subunit gamma; Laminin-9 subunit gamma; Laminin-10 subunit gamma; Laminin-11 subunit gamma; S-laminin subunit gamma |
UniProt | P11047 |
Immunogen | The original rat IgG2a version of this antibody was raised by immunizing fisher rats with high molecular mass material from the Engelbreth-Holm-Swarm (EHS) tumor matrix containing laminin, entactin and HSPG, and fusing the spleen cells of these rats with mouse myeloma X63 Ag8.653 cells. |
Application Notes | This original rat IgG2a clone can be used to detect and analyze the presence of laminin. The specificity towards this protein has been confirmed using immunoprecipitation (Ljubimov et al., 1986; PMID： 3522258). The location of the laminin was confirmed to be in the basment membrane by means of immunofluorescence using this antibody (Eriksdotter-Nielson et al., 1986; PMID： 3537540). |
Antibody First Published | Ljubimov AV, Afanasjeva AV, Litvinova LV, Senin VM Basement membrane components produced by a mouse ascites teratocarcinoma TB24. Analysis with monoclonal and polyclonal antibodies Exp Cell Res. 1986 Aug;165(2)：530-40 PMID:3522258 |
Note on publication | Describes the generation and characterization of the antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
