Key features and details | |
Cat. No. | MABL-2242 |
Name | Anti-LDL Receptor mAbs |
Clone No. | AFD- C7 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | inhibition, IP, WB, FC, IF |
Species Reactivity | Bovine, Human |
Basic Information | |
Specificity | The antibody recognizes the ligand binding domain. It binds to the bovine LDL-R, the human LDL-R but it does not cross react with the LDL-Rs of rat, mouse, Chinese hamster, rabbit and dog. The LDL receptor is a cell surface protein that binds LDL, the major cholesterol-transport protein in plasma, and mediates its endocytosis. |
Alternative Name | LDLR; Low-density lipoprotein receptor; IgG-C7 |
UniProt | P01130 |
Immunogen | The original antibody was produced by immunization of BALB/C mice with the extra cellular domain of the bovine LDL-R. |
Application Notes | The antibody (IgG2b) was employed for the staining of the LDL receptor in normal fibroblast. The antibody competed with LDL for binding to the LDL receptor of intact fibroblast; complete inhibition was achieved at 37 C but not at 4 C (Beisiegel et al., 1981; PMID: 6271765). The antibody detected LDL receptor from human fibroblasts and bovine adrenal cortex by western blot analysis (Beisiegel et al., 1982; PMID: 6290495). The LDL receptor was immunoprecipitated using this antibody (Davis et al., 1987; PMID: 3494949). This antibody was used for detection of LDL receptor expressed on Chang and CHO cells by flow cytometry (Holst et al., 2001; PMID: 11781697) (Ranheim et al., 2006; PMID:16740646). |
Antibody First Published | Beisiegel et al. Monoclonal Antibodies to the Low Density Lipoprotein Receptor as Probes for Study of Receptor-mediated Endocytosis and the Genetics of Familial Hypercholesterolemia J Biol Chem. 1981 Nov 25;256(22):11923-31. PMID:6271765 |
Note on publication | The paper describes the generation and characterization of the antibody. The antibody was used as a probe for the study of the cellular and genetic factors involved in receptor-mediated endocytosis. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
