Key features and details | |
Cat. No. | MABL-2249 |
Name | Anti-LeX mAbs |
Clone No. | AFD- 291-2G3-A |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ICC, IHC |
Species Reactivity | n/a |
Basic Information | |
Specificity | The antibody binds to Lewis X trisaccharide, a carbohydrade pivotal in mediating specific cell- cell interactions, with an affinity of 11 μM. |
Alternative Name | Sialyl Lewis X; SLeX; Stage-specific embryonic antigen 1; SSEA-1; CD15 |
UniProt | |
Immunogen | This antibody was obtained from mice infected with schistosomes. |
Application Notes | The antibody binds specifically to Lewis X, a tetrasaccharide carbohydrate blood-group antigen involved in cell-to-cell adhesion. The antigen is involved in human fertilisation, as it is present on the terminus of N- and O-glycans of the zona pellucida of egg cells, which are recognised by sperm cells This interaction allows fusion and fertilisation to occur. LeX also plays a role in leukocyte tethering as they travel through the bloodstream as it can interact with L-selectin, P-selectin and E-selectin. LeX is also present on neutrophils, mediating phagocytosis and chemotaxis, and is expressed in patients with Hodgkin disease, B- cell chronic lymphocytic leukemia, acute lymphoblastic leukemias and acute nonlymphocytic leukemia. It is also expressed as SSEA-1 on mouse embryonic stem cells. LeX also plays a role in human resistance to the parasitic disease schistosomiasis. |
Antibody First Published | van Roon AM, Pannu NS, Hokke CH, Deelder AM, Abrahams JP. Crystallization and preliminary X-ray analysis of an anti-Lewis X Fab fragment with and without its Lewis X antigen. Acta Crystallogr D Biol Crystallogr. 2003 Jul;59(Pt 7):1306-9. Epub 2003 Jun 27. PMID:12832795 |
Note on publication | Describes the production of the Fab fragment of the monoclonal antibody 291-2G3- A, used to crystallise the structure of Lewis X. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
