Anti-Lewis a,b mAbs

Cat. No.

MABL-2248

Name

Anti-Lewis a,b mAbs

Clone No.

AFD- HEA164

From

Recombinant Antibody

Isotype

Engineer antibody

Application

IP, WB, FC, IF, IHC

Species Reactivity

Human

Basic Information

Specificity

This antibody is specific for Lewis a,b   carbohydrate antigens.

Alternative Name

Blood Group Lewis a/b; lewis a; lewis b

UniProt

Immunogen

This antibody was raised by immunising mice   with human carcinoma cells.

Application Notes

This antibody has applications in the detection   of normal and neoplastic epithelial cells. This antibody has been shown to  react with a glycolipid fraction isolated from colon carcinoma tissue, with   no effect of pronase E addition on its reactivity (Moldenhauer et al, 1987).   This antibody, when immobilised on the wall of a shear chamber, has been   shown to support slow leukocyte rolling (Chen et al, 1997).

Antibody First   Published

Moldenhauer et al. Epithelium-specific   surface glycoprotein of Mr 34,000 is a widely distributed human carcinoma   marker. Br J Cancer. 1987 Dec; 56(6): 714–721. PMID:2449234

Note on publication

Describes the original use of this antibody.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.