Key features and details | |
Cat. No. | MABL-2247 |
Name | Anti-Lewis A mAbs |
Clone No. | AFD- PR 5C5 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA, IF, IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody recognises human Lewis A antigen, and cross-reacts weakly with Galα1-4Gal and rhamnose. |
Alternative Name | LE; FUT3; FUT2; ABO; PR.5C5; pr.5c5 |
UniProt | |
Immunogen | This antibody was raised by immunising BALB/c mice with mucosal scrapings and cell membrane preparations from normal human large intestine. |
Application Notes | This antibody recognises human Lewis A antigen, and has been employed in the immunohistochemical diagnosis of colon disorders, including bowel, small intestine, stomach and colorectal cancers. It has been used in indirect immunoperoxidase staining of formalin-fixed, paraffin-embedded samples of normal colorectal epithelial cells and colon carcinomas (Richman & Bodmer, 1987). It has also been used in indirect immunofluorescence examination of frozen sections of colorectal tissue (Richman & Bodmer, 1987). Its specificity has been confirmed in ELISA analysis (Manimala et al, 2007). |
Antibody First Published | Richman & Bodmer Monoclonal antibodies to human colorectal epithelium: markers for differentiation and tumour characterization. Int J Cancer. 1987 Mar 15;39(3):317-28. PMID:2434440 |
Note on publication | Describes the original generation of this antibody, and its use in immunohistochemistry experiments |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
