Anti-Lipid A mAbs

Cat. No.

MABL-2257

Name

Anti-Lipid A mAbs

Clone No.

AFD- S1-15

From

Recombinant Antibody

Isotype

Engineer antibody

Application

crystallization, EIA, PIH, ELISA

Species Reactivity

Gram-negative bacteria

Basic Information

Specificity

The antibody recognizes the carbohydrate   backbone of lipid A. The antibody binds to the 4'- monophosphoryl GlcN   disaccharide. The antibody does not cross react with lipopolysaccharides   (LPS). The antibody cross reacts with ssDNA. Lipid A is the lipid component   of LPS contained in gram-negative bacteria. Lipid A is the endotoxic   principle of LPS.

Alternative Name

lipA

UniProt

Immunogen

The original antibody was generated by   immunizing BALB/c mice with lipA.

Application Notes

In passive immunohemolysis, the antibody reacted   with bisphosphoryl compounds as well as with the 4'-monophosphoryl partial   structures (Kuhn et al., 1992; PMID 1375194). The specificity of the antibody   was determined by ELISA analysis. The crystal structures of the Fab fragment in complex with lipid A carbohydrate backbone and in the unliganded form were   determined by X-ray diffraction, showing the binding mechanism of the   antibody is a lock and key type. Further, the structure of the Fab fragment   in complex with single-stranded DNA fragment 5(dT) oligonucleotide was   determined. The antibody bound two separate oligonucleotides by their   terminal 5- and 3-phosphate groups (Haji-Ghassemi et al., 2015; PMID:   26085093). Solid-phase enzyme immunoassay shown the antibody binds better to   the glycoconjugate at low antigen concentrations and better to the lipid A   when high concentrations of antigen were used (Brade et al., 1993).

Antibody First   Published

Kuhn et al. Characterization of the epitope specificity of murine monoclonal antibodies directed against lipid A. Infect Immun. 1992   Jun;60(6):2201-10. PMID:1375194

Note on publication

The paper describes the generation and   characterization of the antibody.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.