Key features and details | |
Cat. No. | MABL-2258 |
Name | Anti-Lipopolysaccharide mAbs |
Clone No. | AFD- S69-4 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | SPR, TLC, ELISA, IF |
Species Reactivity | Chlamydophila psittaci |
Basic Information | |
Specificity | This antibody is specific for Kdo4, a Chlamydophila psittaci Lipopolysaccharide (LPS) tetrasaccharide (3-deoxy-alpha-d-manno-oct-2-ulopyranosonic acid (Kdo)) of the sequence Kdo(2-->8)[Kdo(2-->4)] Kdo(2-->4)Kdo. This antibody specifically binds to the LPS of Chlamydophila psittaci but not to LPS from Chlamydophila pneumoniae or Chlamydia trachomatis. Furthermore, it binds exclusively to the LPS and O-deacylated LPS of Chlamydophila psittaci. |
Alternative Name | LPS; Kdo(2→8)[Kdo(2→4)]Kdo(2→4) Kdo; Kdo4; branched Kdo4 tetrasaccharide; Chlamydophila psittaci Lipopolysaccharide tetrasaccharide; 3-deoxy-alpha-d-manno-oct-2- ulopyranosonic acid |
UniProt | |
Immunogen | The original antibody was generated by immunizing BALB/c mice with a synthetic neoglycoconjugate antigen — Kdo(2-->8)[Kdo(2-->4)]Kdo(2-->4) Kdo-BSA (Kdo4-BSA). |
Application Notes | This antibody demonstrated high affinity and specificity for structures containing the branched Kdo4 tetrasaccharide or the 2→4/2→4Kdo3 trisaccharide, enabling differentiation of Chlamydophila psittaci from other chlamydial species. Furthermore, it shows potential for detecting individual molecular lipopolysaccharide (LPS) species and studying the enzymology of Kdo transferases. Its binding affinity to neoglycoconjugate antigens was assessed using ELISA checkerboard titrations. It displayed comparable affinity for 2→4/2→4Kdo3-BSA and Kdo4-BSA and weak binding was observed with 2→4Kdo2-BSA, 2→8Kdo2-BSA, and 2→8/2→4Kdo3-BSA, but only at high antibody and antigen concentrations. This antibody was also tested against BSA glycoconjugates containing 4'-monophosphoryl derivatives of deacylated C. psittaci LPS. It demonstrated similar reactivity with 2→4/2→4Kdo3-GlcNAc2-4P-BSA and Kdo4- GlcNAc2-4P-BSA. |
Antibody First Published | Müller-Loennies et al. A monoclonal antibody against a carbohydrate epitope in lipopolysaccharide differentiates Chlamydophila psittaci from Chlamydophila pecorum, Chlamydophila pneumoniae, and Chlamydia trachomatis Glycobiology. 2006 Mar;16(3):184-96. doi: 10.1093/glycob/cwj055 PMID:16282606 |
Note on publication | The original publication discusses the development of a monoclonal antibody that can differentiate Chlamydophila psittaci from other Chlamydophila species and Chlamydia trachomatis based on a specific carbohydrate epitope in the lipopolysaccharide. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
