Anti-Ly6G/Ly6C mAbs

Cat. No.

MABL-2287

Name

Anti-Ly6G/Ly6C mAbs

Clone No.

AFD- RB6-8C5

From

Recombinant Antibody

Isotype

Engineer antibody

Application

deplete, WB, FC, IHC

Species Reactivity

Mouse

Basic Information

Specificity

This antibody is specific for murine Ly6G, a a 21-25 kDa glycosylphosphatidylinositol (GPI)- linked protein expressed by  the myeloid-derived cells in a tightly developmentally-regulated manner in   the bone marrow. Monocytes express Ly6G transiently during bone marrow   development, while Ly6G expression in granulocytes and peripheral neutrophils   directly correlates with the cell’s level of differentiation and maturation.   This hallmark makes Ly6G a good marker for these particular cell populations.   Ly6G has also been implicated in the development of antitumor responses.

Alternative Name

Gr-1; Gr1; Ly-6G; ly-6G.1; ly6C

UniProt

P35461

Immunogen

This antibody was raised in Lou-M rats using   a popliteal lymph node immunisation and fusion protocol as described by   Martiniello et al (1994)

Application Notes

This antibody has been used in FACS   analyses, immunoprecipitation, immunoblotting, and immunohistochemistry in   various studies, such as to elucidate the origin and roles of macrophages in   the post-myocardial infarction remodelling (Sager et al, 2016), to   investigate the pathogenesis of primary biliary cirrhosis (Yang et al, 2016),   to confirm the phenotypic dynamics of microglial and monocyte-derived cells   in glioblastoma-bearing mice (Ricard et al, 2016), and to highlight an important ageing-related defect in the mucosal immune system's ability to   sample lumenal antigens (Kobayashi et al, 2013). This antibody has also been   used in vivo, for example, to deplete Treg cells when delineating the   paralleled developments of inflammation and immunosuppression in tumor   microenvironment (Yang et al, 2010), or to deplete neutrophils when   underpinning a causal role for neutrophils in severe asthma (Wilson et al,   2009).

Antibody First   Published

Stephen Hurst et al. New IL-17 family   members promote Th1 or Th2 responses in the lung: in vivo function of the   novel cytokine IL-25. J Immunol. 2002 Jul 1;169(1)11:10:443-53. PMID:12077275

Note on publication

Describe the use of this antibody for FACS   analysis and in vivo cell depletion to demonstrate the function of the novel   cytokine IL-25 in the lung.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.