Key features and details | |
Cat. No. | MABL-2286 |
Name | Anti-Ly6G mAbs |
Clone No. | AFD- 1A8 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Depletion, FC |
Species Reactivity | Mouse |
Basic Information | |
Specificity | Recognises the murine GPI-anchored protein Ly6G, a marker of granulocyte differentiation, maturation and, together with Ly6C, a component of the Granulocyte Receptor-1 Antigen (Gr-1). Unlike another commonly used clone, RB6-8C5, 1A8 does not bind Ly6C. |
Alternative Name | LGR-1 ; y-6G; GR1; Lymphocyte antigen 6G |
UniProt | P35461 |
Immunogen | LEW/N rats immunized with 1.5x10E8 Ly-6G-transfected EL4J cells |
Application Notes | Neutrophils are crucial components of our immune system. Ly6G is highly expressed on neutrophils, which allows the 1A8 clone to be used not only in detection of neutrophils, but also in selective depletion |
Antibody First Published | Fleming TJ, Fleming ML, Malek TR. Selective expression of Ly-6G on myeloid lineage cells in mouse bone marrow. RB6-8C5 mAb to granulocyte-differentiation antigen (Gr-1) detects members of the Ly-6 family. Journal of Immunology PMID:8360469 |
Note on publication | Describes production of 1A8 and identification of Ly6G as its target. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
