Key features and details | |
Cat. No. | MABL-2285 |
Name | Anti-Ly-6C (T cell activating) mAbs |
Clone No. | AFD- HK1.4 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | functional assay, IP, FC, IF |
Species Reactivity | Mouse |
Basic Information | |
Specificity | This antibody is specific for Ly6 and shows no cross reactivity. Ly6 shows acetylcholine receptor binding activity. |
Alternative Name | Lymphocyte antigen 6C1; Ly6c1 |
UniProt | P0CW02 |
Immunogen | The antibody was raised by immunizing a Lewis rat by a series of four monthly i.p. injections of approximately 30 X 10" L3 cloned CTL cells. |
Application Notes | A flow cytometry experiment on mouse cells from L-gD-1, an L cell line that expresses HSV type 1 gD, and L-gI-1, an L cell line that expresses HSV type 1 gI was preformed. In this experiment this antibody was used for reverse blocking (Johnson et al, 1993; pmid:8104217). Th1 clones from either wild- type or fyn-/- mice were used in an experiment where it was determined if this antibody could induce lysis (Lancki et al, 1995; pmid:7493751). Flow cytometry was preformed on cells from C57BL/6, DBAI2, NJ. BALB/c, PIJ, PL/J. AKRIJ. RIII, SM/J, SJL, and CBA mice using this antibody (Havran et al, 1988; pmid:3257767). Immunofluorescence was preformed on rat EL4J cells using this antibody (Fleming et al, 1993; pmid:8360469). EL4J and EUJ-Ly-6G cell lines were immunoprecipitated with this antibody (Fleming et al, 1993; pmid:8360469). |
Antibody First Published | Havran et al. Characterization of an anti-Ly-6 monoclonal antibody which defines and activates cytolytic T lymphocytes J Immunol. 1988 Feb 15;140(4):1034-42. PMID:3257767 |
Note on publication | The paper describes the generation of the antibody is rats by immunizing them with L3 cloned CTL cells. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
