Anti-Lysozyme mAbs

Cat. No.

MABL-2293

Name

Anti-Lysozyme mAbs

Clone No.

AFD- cAbHul6

From

Recombinant Antibody

Isotype

Engineer antibody

Application

crystallization, SPR

Species Reactivity

Human

Basic Information

Specificity

The antibody is specificity for human   lysozyme. It does not cross react with hen lysozyme.

Alternative Name

1,4-beta-N-acetylmuramidase C; cAb-Hul6; NbHuL6

UniProt

P61626

Immunogen

Phage libraries were generated using the mRNA   extracted from the lymphocytes of immunized dromedaries. The VHH antibody was   isolated by biopanning from the libraries.

Application Notes

The binding affinity of the VHH fragment for   human lysozyme was measured by surface plasmon resonance (Kd = 0.7 nM)   (Dumoulin et al., 2002; PMID: 11847273). The antibody stabilized an unstable   human lysozyme mutant that forms fibrils and amyloids.The crystal structure   of the original version of the antibody in complex with human lysozyme was   determined (Dumoulin et al., 2003; PMID: 12917687). The original antibody was   humanized and the binding affinity for human lysozyme was measured by surface   plasmon resonance (Kd = 2.36 nM). The crystal structure of the humanized   version of the antibody in complex with human lysozyme was determined (Vincke   et al., 2009; PMID:19010777).

Antibody First   Published

Dumoulin et al. Single-domain antibody   fragments with high conformational stability. Protein Sci. 2002 Mar;11(3); 500-15. do:10.1110/ps.34602. PMID:11847273

Note on publication

The original paper describes the generation   and characterization of the antibody

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.