Key features and details | |
Cat. No. | MABL-2294 |
Name | Anti-Lysozyme mAbs |
Clone No. | AFD- D1.3 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA, FC |
Species Reactivity | Chicken |
Basic Information | |
Specificity | This antibody specifically binds hen egg-white lysozyme. |
Alternative Name | HEL; Hen Egg white Lysozyme; Lysozyme C; 1,4-beta-N- acetylmuramidase C; Allergen Gal d IV; Allergen: Gal d 4 |
UniProt | P00698 |
Immunogen | The original antibody was generated by immunizing BALB/c mice with hen egg white lysozyme. |
Application Notes | Lysozyme is an enzyme that degrades polysacchardies of various types of bacterial cell walls. It is observed that lysozyme bound D1.3 still retains its enzymatic activity. This is attributed to the fact that D1.3 binds lysozyme at a site remote from the catalytic site and that there is no significant conformational change caused by the binding (PMID: 3614217). The three dimension structure of a complex between the Fab from antibody D1.3 and its antigen, HEL, has been determined at 6 and at 2.8 a resolution (PMID: 2426778, 3965976). Sixteen residues of lysozyme make contact with 17 residues of the antibody. The antigenic determinant is topographic including lysozyme residues 18, 19, 21–25, 27, 116–121, 124, and 129. All six complementarity determining regions of the antibody contact the antigen, and in addition, two residues from “framework” segments of V H and V L (Amit et al., 1986; PMID: 2441250). D1.3 binds native lysozyme in an ELISA (PMID: 2448386). |
Antibody First Published | Mariuzza et al. Preliminary Crystallographic Study of the Complex Between the Fab Fragment of a Monoclonal Anti-Lysozyme Antibody and Its Antigen. J Mol Biol, (1983) 15;170(4):1055-8. PMID:6644814 |
Note on publication | Describes the crystallographic study of the monoclonal antibody bound to lysozyme. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
