Anti-Macaque pan-species IgG mAbs

Cat. No.

MABL-2314

Name

Anti-Macaque pan-species IgG mAbs

Clone No.

AFD- 1B3

From

Recombinant Antibody

Isotype

Engineer antibody

Application

ELISA, FC

Species Reactivity

Baboon, Pigtailed Macaque, rhesus macaque,   Cynomolgus Monkey

Basic Information

Specificity

This antibody reacts with rhesus macaque   IgG1 and IgG3, as well a cynomolgus monkey, pigtailed macaque and baboon IgG.   It has minimal reactivity with human, mangabey, vervet, chimpanzee, or   marmoset IgG.

Alternative Name

Macaque IgG; Macaque immunoglobulin G

UniProt

Immunogen

This antibody was raised by immunising mice   with baboon IgG and screening hybridomas against IgG of different primate   species.

Application Notes

This anti-macaque antibody has been used in   ELISA analysis to detect rhesus anti- mouse antibodies in serum samples (Page   et al, 2012). As this antibody is able to distinguish between human and   macaque IgG, it has also been used in ELISA analysis to detect macaque   antibodies raised against intravenously-administered human HIV broadly   neutralising antibodies (Rosenberg et al, 2015). Additionally, it has been   used in flow cytometric analysis to confirm the clearance of intravenously-   administered anti-CD4 depleting antibody from the plasma of rhesus macaques   (Mudd et al, 2011).

Antibody First   Published

Mudd et al. Reduction of CD4+ T cells in   vivo does not affect virus load in macaque elite controllers. J Virol. 2011   Jul;85(14):7454-9. PMID:21593153

Note on publication

This antibody is from the laboratory of   Keith Reimann, DVM, Beth Israel Deaconess Medical Center.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.