Key features and details | |
Cat. No. | MABL-2313 |
Name | Anti-MAC-1 mAbs |
Clone No. | AFD- YBM15.1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | FC, IHC |
Species Reactivity | Mouse |
Basic Information | |
Specificity | This antibody recognizes murine integrine MAC-1 (Itgam/Cd11b). This integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens. |
Alternative Name | CD11b; Integrin alpha-M; CD11 antigen-like family member B; CR-3 alpha chain; Cell surface glycoprotein MAC-1 subunit alpha; Leukocyte adhesion receptor MO1; YBM 15.1.6; YBM15.1.6 |
UniProt | P05555 |
Immunogen | This antibody was derived from fusions between the 210 RC Y3/Ag 1.2.3 rat myeloma cell line and spleen cells from rats hyperimmunized with adult mouse bone marrow. |
Application Notes | This antibody is recommended for the detection and analysis of MAC-1, the murine equivalent of CD11b. For instance, YBM15.1 was used in a panel of antibodies in the assessment of the presence of antigenic determinants on normal erythroid progenitors via flow cytometry (Watt et al., 1987; pmid: 3330997). This antibody was used to detect the integrin MAC-1 in the neural tissue of Biozzi ABH mice infected with Semliki Forest virus (SFV) A7(74) via immunohistochemistry (Smith et al., 2000; pmid: 10814783). |
Antibody First Published | Watt et al. Cell-surface markers on haemopoietic precursors. Reagents for the isolation and analysis of progenitor cell subpopulations Mol Cell Probes. 1987 Dec;1(4):297-326. doi: 10.1016/0890-8508(87)90013-2. PMID:3330997 |
Note on publication | The article describes the generation and characterization of this antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
