Anti-M2 mAbs

Cat. No.

MABL-2312

Name

Anti-M2 mAbs

Clone No.

AFD- C2

From

Recombinant Antibody

Isotype

Engineer antibody

Application

FC, IF

Species Reactivity

Influenza A

Basic Information

Specificity

The antibody is specific for   M2-cytoplasmatic domain. M2 is a 97-amino acid protein of which approximately   half (aa 47–97) comprises the cytoplasmic domain. M2 is highly conserved   among virus strains.

Alternative Name

M2 protein; M; Matrix protein 2; Proton channel protein M2; M2-E10

UniProt

P05780

Immunogen

The original antibody was generated by phage   display and yeast display based antibody selection and screening. The   purified M2 cytodomain was biotinylated and used as a target for phage   display-based single-chain variable fragment (scFv) antibody selection. After   two rounds of phage selection, scFv genes were cloned into a yeast display   vector, and the yeast display library was sorted for binding.

Application Notes

The scFv-Fc version of the antibody was   tested against HEK cells expressing full-length M2 protein. Fluorescent   signals were visible lining the internal cell wall of HEK M2 cells after   fixation and permeabilization. No signal was visible in HEK cells that do not   express M2, or when the cells were not permeabilized. The ability of the   antibody to recognize native M2 protein produced during IVA infection of MDCK   cells with the H3N2 (A/PCh/1/73) and H1N1 (A/PR/8/34) strains of influenza   was detected. Efficient and specific recognition of native M2 protein by the   scFv-Fc fragment of the antibody was observed via fluorescent microscopy and   flow cytometry (Velappan et al., 2020; PMID: 33206590).

Antibody First   Published

Velappan et al. Selection and verification   of antibodies against the cytoplasmic domain of M2 of influenza, a   transmembrane protein MAbs. Jan-Dec 2020;12(1):1843754. PMID:3320659

Note on publication

The original paper describes the generation   of the original antibody, using phage and yeast display selection techniques.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.