Key features and details | |
Cat. No. | MABL-2317 |
Name | Anti-MAdCAM-1 mAbs |
Clone No. | AFD- MECA-89 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Block, IHC |
Species Reactivity | Mouse |
Basic Information | |
Specificity | The antibody is specific for mouse MAdCAM-1. The antibody recognizes the second immunoglobulin domain, interfering with L-selectin interactions of MAdCAM-1. The mucosal lymphocyte homing receptor MAdCAM-1 is expressed in the high endothelial venules of the mesenteric lymph nodes (MLN) and Peyer's patches (PP) and in the flat epithelium of the lamina propria (LP). |
Alternative Name | ATCC HB-292; Addressin; mucosal vascular addressin cell adhesion molecule 1 |
UniProt | |
Immunogen | Endothelial cells isolated from BALB/c mouse mesenteric and peripheral lymph nodes. |
Application Notes | The antibody bound to the second domain of MAdCAM-1 and did not block MAdCAM-1- dependent binding in vitro (Streeter et al., 1988; PMID:3340147). The antibody was used for immunohistochemical analysis of healthy AKR control mice and it showed faint basal expression of MAdCAM- 1 in the terminal ileum (Bachmann et al. 2006). The antibody blocked interactions with L-selectin in vivo (Bargatze et al., 1995; PMID: 7542550). |
Antibody First Published | Streeter et al. A tissue-specific endothelial cell molecule involved in lymphocyte homing. Nature. 1988 Jan 7;331(6151):41-6. PMID:3340147 |
Note on publication | The original paper describes the role of MAdCAM-1 in lymphocyte homing. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
