Anti-mCherry mAbs

Cat. No.

MABL-2333

Name

Anti-mCherry mAbs

Clone No.

AFD- LAM-2

From

Recombinant Antibody

Isotype

Engineer antibody

Application

ELISA, IF

Species Reactivity

Discosoma sp. LW-2004

Basic Information

Specificity

This antibody specifically binds mCherry   fluorescent protein.

Alternative Name

mCh; RFP; Fluorescent protein raspberry; Red   fluorescent protein; RFP- RANbody-2; LaM2

UniProt

Q5S3G8

Immunogen

The original antibody was generated by   immunization of Llama glama with mCherry fluorescent protein. A VHH cDNA   library was generated by high-throughput DNA sequencing of a marrow   lymphocyte from an immunized llama. Later on this was combined with mass spectrometric   (MS) identification of high affinity VHH regions derived from serum of the   same animal.

Application Notes

This antibody is specific for mCherry and   does not cross react with DsRed or TdTomato. This antibody can be used to   detect mCherry fluorescent protein in an ELISA. This antibody binds   immobilized mCherry with a binding affinity of Kd= 0.49 nM (PMID: 25362362).

Antibody First   Published

Fridy et al. A robust pipeline for rapid   production of versatile nanobody repertoires. Nat Methods. 2014 Dec; 11(12):   1253–1260. PMID:25362362

Note on publication

Describes the generation of large   repertoires of readily expressible recombinant nanobodies with high affinities   and specificities against a given antigen.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.