Anti-mCherry mAbs

Cat. No.

MABL-2334

Name

Anti-mCherry mAbs

Clone No.

AFD- LAM-4

From

Recombinant Antibody

Isotype

Engineer antibody

Application

ELISA, IF, IHC

Species Reactivity

Anaplasma marginale, Discosoma sp. LW-2004

Basic Information

Specificity

This antibody binds mCherry and can also   bind DsRed.

Alternative Name

mCh; RFP; Fluorescent protein raspberry; Red   fluorescent proteins; MCherry fluorescent protein; DsRed; RFP-RANbody-4;   LaM4; RFP nanobody; RNb

UniProt

Q5S3G8; X5DSL3

Immunogen

The original antibody was generated by   immunization of Llama glama with mCherry fluorescent protein. A VHH cDNA   library was generated by high-throughput DNA sequencing of a marrow   lymphocyte from an immunized llama. Later on this was combined with mass   spectrometric (MS) identification of high affinity VHH regions derived from   serum of the same animal.

Application Notes

This antibody can be used to detect mCherry   fluorescent protein and DsRed in an ELISA. This antibody binds immobilized  mCherry with a binding affinity of Kd= 0.18 nM. The Alexa Fluor® 488   conjugated version of this antibody was used to immunofluorescently stain S.   cerevisiae expressing mCherry-tagged histone H2B (PMID: 25362362). This   antibody was used for the visualization and manipulation of red fluorescent   labelled proteins in live cells by using nanobodies targeting fluorescent   protein tags (PMID: 31122229).

Antibody First   Published

Fridy et al. A robust pipeline for rapid   production of versatile nanobody repertoires. Nat Methods. 2014 Dec; 11(12):   1253–1260. PMID:25362362

Note on publication

Describes the generation of large   repertoires of readily expressible recombinant nanobodies with high   affinities and specificities against a given antigen.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.