Key features and details | |
Cat. No. | MABL-2334 |
Name | Anti-mCherry mAbs |
Clone No. | AFD- LAM-4 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA, IF, IHC |
Species Reactivity | Anaplasma marginale, Discosoma sp. LW-2004 |
Basic Information | |
Specificity | This antibody binds mCherry and can also bind DsRed. |
Alternative Name | mCh; RFP; Fluorescent protein raspberry; Red fluorescent proteins; MCherry fluorescent protein; DsRed; RFP-RANbody-4; LaM4; RFP nanobody; RNb |
UniProt | Q5S3G8; X5DSL3 |
Immunogen | The original antibody was generated by immunization of Llama glama with mCherry fluorescent protein. A VHH cDNA library was generated by high-throughput DNA sequencing of a marrow lymphocyte from an immunized llama. Later on this was combined with mass spectrometric (MS) identification of high affinity VHH regions derived from serum of the same animal. |
Application Notes | This antibody can be used to detect mCherry fluorescent protein and DsRed in an ELISA. This antibody binds immobilized mCherry with a binding affinity of Kd= 0.18 nM. The Alexa Fluor® 488 conjugated version of this antibody was used to immunofluorescently stain S. cerevisiae expressing mCherry-tagged histone H2B (PMID: 25362362). This antibody was used for the visualization and manipulation of red fluorescent labelled proteins in live cells by using nanobodies targeting fluorescent protein tags (PMID: 31122229). |
Antibody First Published | Fridy et al. A robust pipeline for rapid production of versatile nanobody repertoires. Nat Methods. 2014 Dec; 11(12): 1253–1260. PMID:25362362 |
Note on publication | Describes the generation of large repertoires of readily expressible recombinant nanobodies with high affinities and specificities against a given antigen. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
