Key features and details | |
Cat. No. | MABL-2370 |
Name | Anti-MHC II mAbs |
Clone No. | AFD- OX-6 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ICC, IP, RIA, WB, FC, IF, IHC |
Species Reactivity | Rat, Mouse |
Basic Information | |
Specificity | This antibody recognises a monomorphic determinant of the rat MHC II (I-A) antigen present on B lymphocytes, dendritic cells, some macrophages and certain epithelial cells. It also cross-reacts with mouse MHC, specifically I-A[k] and I-A[s] |
Alternative Name | MHC Class II; MRC OX-6; CD74; MRC OX 6; Major Histocompatibility complex II |
UniProt | |
Immunogen | This antibody was prepared by subcutaneously injecting non-polymorphic MHC class II (Ia-like glycoproteins) from the membranes of rat thymocytes into BALB/c mice. |
Application Notes | This antibody binds to the rat MHC II (I-A) antigen, and cross-reacts with mouse I-A[k] and I-A[s]. Consequently, this antibody can be used to assess the tissue distribution of rat Ia antigens, indicating high Ia antigen expression on peripheral B lymphocytes, and significantly lower expression on thymocytes and T lymphocytes (McMaster, 1979). |
Antibody First Published | McMaster et al. Identification of Ia glycoproteins in rat thymus and purification from rat spleen. European Journal of Immunology. 1979, 9: 426-433 PMID:315315 |
Note on publication | Describes the original use of this antibody to identify and purify rat Ia glycoproteins, using indirect binding assays, FC and IP. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
