Anti-MHCII mAbs

Cat. No.

MABL-2375

Name

Anti-MHCII mAbs

Clone No.

AFD- VHH7

From

Recombinant Antibody

Isotype

Engineer antibody

Application

Blocking, in vitro, in vivo, IP, SPR, FC,   IF, Imaging

Species Reactivity

Mouse

Basic Information

Specificity

This antibody is specific for mouse MHCII.

Alternative Name

class II MHC; class II histocompatibility   antigen

UniProt

Immunogen

The original antibody was generated by immunizing an alpaca with intact, unfractionated mouse splenocytes seven   times.

Application Notes

This nanobody was linked to an anti-GFP VHH via their C termini to create a bispecific antibody capable of targeting both GFP and MHC class II Molecules; the VHH7-GFP bispecific construct effectively   targeted B cells and facilitated the uptake of GFP by these cells, as   indicated by a flow cytometry (FC) analysis that showed that most CD19+ cells   were GFP+, indicating successful capture of GFP in vivo.The same bispecific   antibody was used to stain B cells in mouse lymph node cells, as observed   using FC. This nanobody was also used to C-terminally conjugate human IL2 and   IFN-α to anti-GFP, demonstrating the general applicability of the approach   for linking different entities (Witte et al., 2012)

Antibody First   Published

 Duarte et al. Generation of Immunity against   Pathogens via Single-Domain Antibody-Antigen Constructs J Immunol. 2016 Dec   15;197(12):4838-4847. doi: 10.4049/jimmunol.1600692 PMID:27821668

Note on publication

The original publication describes the   development and application of single-domain antibody fragments (VHHs), such   as VHH7, as targeted antigen-delivery vehicles for enhancing immune responses   in antigen-presenting cells.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.