Key features and details | |
Cat. No. | MABL-2376 |
Name | Anti-Migis-alpha mAbs |
Clone No. | AFD- 29C11 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, ELISA |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody binds the extracellular peptide called the Migis-α consisting of 26-32 amino acids towards the N-terminal of the human membrane bound IgA, when the lipid rafts are disrupted. |
Alternative Name | Migis-α; mIgA isotype-specific extracellular peptide; extracellular membrane proximal domain of mIgA α chain |
UniProt | |
Immunogen | The original antibody was generated by immunizing BALB/c mice with a mouse/human chimeric migis-αL-containing protein. |
Application Notes | Membrane bound IgA is associated with Igα/Igβ as the B cell receptor (BCR) complex on mIgA-expressing B cells. The α chain of mIgA (mα) contains an extracellular domain that is reported to be an antigenic site suitable for isotype-specific targeting of mIgA-expressing B cells by antibodies. The mAb binds strongly to synthetic peptides of migis-α and to various recombinant proteins containing migis-α as revealed by ELISA. Flow cytometric analysis with B cells suggested that the mAb did not bind strongly to mIgA and required a higher concentration for detection. Immunoprecipitation analysis of these mAbs indicated that mIgA could only be pulled down by 29C11 when mIgA-expressing B cells were solubilized by strong detergents, such as sodium dodecyl sulfate (SDS) or when lipid rafts are disrupted (PMID: 21723611). |
Antibody First Published | Hung et al. Lipid rafts hinder binding of antibodies to the extracellular segment of the membrane-anchor peptide of mIgA. Molecular Immunology (2011) Sep ;48 (15-16):1975-82. PMID:21723611 |
Note on publication | Describes the generation and characterization of this antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
