Key features and details | |
Cat. No. | MABL-2393 |
Name | Anti-Mouse Nidogen/enactin mAbs |
Clone No. | AFD- JF4 (5G12-D4-E3) |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | functional assay, SPR, WB, ELISA, FC, IF, IHC |
Species Reactivity | Human, Mouse |
Basic Information | |
Specificity | This antribody is specific for the G2 domain of nidogen-1. It binds in such a way that it is able to stop the binding of nidogen to laminin. Nidogen-1 is a sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions. |
Alternative Name | Nidogen-1; Nid1; Entactin; NID-1 |
UniProt | P10493 |
Immunogen | This antibody was raised by immunizing a rat with he G2 region of mouse nidogen that is able to block its binding to laminin. |
Application Notes | The binding of the mouse version of this antibody to nidogen-1 was proven by ELISA. Further, high binding affinity of the mouse version of this antibody to nidogen-1 was demonstrated using surface plasmon resonance (Ries et al, 2001; pmid:11589703). While researching nidogen in healthy human cartilage and in late-stage osteoarthritis cartilage an inhibition assay was preformed using the mouse version of this antibody. In short, wells were coated with either nidogen-1 or nidogen-2 to which this antibody was added. Then, chondrocytes were added and their adhesion to the nidogen was tested. 30-40% of chondrocytes showed adhesion to nidogen-1. (Kruegel et al, 2008; pmid:18438862). Tissue sections from Female New Mexico Research Institute (NMRI) mice were stained for immunohistochemistry using this antibody as a positive control. |
Antibody First Published | Ries et al. Recombinant domains of mouse nidogen-1 and their binding to basement membrane proteins and monoclonal antibodies Eur J Biochem. 2001 Oct;268(19):5119-28. PMID:11589703 |
Note on publication | Recombinant proteins were produced of different predicted nidogen-1 domains in mammalian cells and used these proteins for crystallographic and binding epitope analyses. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
