Key features and details | |
Cat. No. | MABL-2415 |
Name | Anti-MUC-5AC mAbs |
Clone No. | AFD- 4-5M1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ICC, IP, WB, ELISA, FC, IF, IHC |
Species Reactivity | Cat, Chicken, Pig, Primate, Rabbit, Rat, Human, Mouse |
Basic Information | |
Specificity | This body is specific for the 5' terminal epitope of the MUC5AC. |
Alternative Name | 45M1; Mucin-5AC; Gastric mucin; Major airway glycoprotein; Tracheobronchial mucin; Mucin-5 subtype AC, tracheobronchial |
UniProt | P98088 |
Immunogen | The original antibody was raised by immunizing mice with human gastric mucin M1. |
Application Notes | The original version of this antibody was used for ELISA on NCI-H292 human pulmonary mucoepidermoid cells (Baginski et al., 2006; PMID:16543607), on BAL fluid (bronchoalveolar lavage fluid) (Phillips et al., 2006; PMID:16507438), and on different fractions of corpus tissue (Nordman et al., 2002; PMID:11988092). This antibody was also used for flow cytometry on human lung cells (Weeden et al., 2017; PMID:28125611), and on H292 cells (Montalbano et al., 2014; PMID:24797786). It was able to stain MUC5AC in both experiments. This antibody was able to stain passage 2 normal human tracheobronchial epithelial (NHTBE) cells for immunofluorescence, it was able to determine MUC5AC production during this experiment (Gray et al., 2004; PMID:14527933). The antibody was also used to stain HT29–18N2 goblet cells in a immunocytochemistry experiment, showing staining of the intracellular mucin stores (Stanley and Phillips., 1999; PMID:10409167). |
Antibody First Published | Stanley and Phillips. Selective secretion and replenishment of discrete mucin glycoforms from intestinal goblet cells Am J Physiol. 1999 Jul;277(1):G191-200. PMID:10409167 |
Note on publication | This original paper studies the effect of cholinergic stimulation on the mucin secretion of goblet cells |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
