Key features and details | |
Cat. No. | MABL-2416 |
Name | Anti-MUC1 mAbs |
Clone No. | AFD- 12D10 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | SPR, ELISA, FC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody is specific for the unsubstituted O-6 position of the Neu5Ac residue of MUC1. The alpha subunit of MUC1 has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack. The beta subunit of MUC-1 contains a C-terminal domain which is involved in cell signaling, through phosphorylations and protein-protein interactions. Modulates signaling in ERK, SRC and NF-kappa-B pathways. In activated T- cells, influences directly or indirectly the Ras/MAPK pathway. |
Alternative Name | Mucin-1; Breast carcinoma-associated antigen DF3; Cancer antigen 15-3; CA 15-3; Carcinoma-associated mucin; Episialin; H23AG; Krebs von den Lungen-6; KL-6; PEMT; Peanut- reactive urinary mucin; PUM; Polymorphic epithelial mucin; PEM; Tumor-associated epithelial membrane antigen; EMA; Tumor-associated mucin; CD227 |
UniProt | P15941 |
Immunogen | This antibody was raised by immunizing female Balb/c mice with MUC1 antigen. |
Application Notes | To characterize this antibody an ELISA was preformed on MUC1 glycorproteins. Furthermore, the binding constant of this antibody was determined by surface plasmon resonance on MUC1 glycoprotein using the mouse version of this antibody. Finally, human breast cancer cells (T-47D), human lung adenocarcinoma cells (Calu-3), human mucoepidermoid carcinoma cells (H292), and human lung adenocarcinoma epithelial cells were used for flow cytometry with the murine version of this antibody (Naito et al, 2017; pmid:30023556). |
Antibody First Published | Naito et al. Generation of Novel Anti-MUC1 Monoclonal Antibodies with Designed Carbohydrate Specificities Using MUC1 Glycopeptide Library. ACS Omega. 2017 Nov 30;2(11):7493-7505. PMID:30023556 |
Note on publication | In this study, using an MUC1 glycopeptide library, we established two novel anti- MUC1 monoclonal antibodies (1B2 and 12D10) with designed carbohydrate specificities. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
