Key features and details | |
Cat. No. | MABL-2417 |
Name | Anti-MUC1 mAbs |
Clone No. | AFD- AR20.5 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | functional assay, RIA, therapeutic, ELISA, FC, IF |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody is specific for human MUC1. The alpha subunit of MUC1 has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack. The beta subunit of MUC-1 contains a C-terminal domain which is involved in cell signaling, through phosphorylations and protein-protein interactions. Modulates signaling in ERK, SRC and NF-kappa-B pathways. In activated T-cells, influences directly or indirectly the Ras/MAPK pathway. Promotes tumor progression. Regulates TP53-mediated transcription and determines cell fate in the genotoxic stress response. Binds, together with KLF4, the PE21 promoter element of TP53 and represses TP53 activity. |
Alternative Name | Mucin-1; Breast carcinoma-associated antigen DF3; Cancer antigen 15-3; CA 15-3; Carcinoma-associated mucin; Episialin; H23AG; Krebs von den Lungen-6; KL-6; PEMT; Peanut- reactive urinary mucin; PUM; Polymorphic epithelial mucin; PEM; Tumor-associated epithelial membrane antigen; EMA; Tumor-associated mucin; CD227 |
UniProt | P15941 |
Immunogen | This antibody was raised by immunizing BALB/c mice with MUC1. |
Application Notes | While creating and characterizing this antibody, an ELISA was preformed on MUC1 and a 23-mer MUC1 peptide, E23 (CPAHGVTSAPDTRPAPGSTAPPA), Furthermore, the specificity of the antibody was confirmed using flow cytometry on MCF-7, BT-20 and 413BCR cell lines using the murine version of this antibody. Further, a chromium release assay was preformed using the murine version of this antibody on ZR75-1 cells. And finally the biodistribution of the mouse version of this antibody was tested. This was done on mice injected with ZR75-1 cancer cells (Qi et al, 2001; pmid:11839249). To assess the ability of this antibody to cure cancer mice inoculated with a Panc02.MUC1 cancer cell line were treated with the mouse version of this antibody. |
Antibody First Published | Qi et al. Characterization of an anti-MUC1 monoclonal antibody with potential as a cancer vaccine. Hybrid Hybridomics. 2001;20(5-6):313-24. PMID:11839249 |
Note on publication | Describes the generation and characterization of this antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
