Key features and details | |
Cat. No. | MABL-2451 |
Name | Anti-N-acetyl-ganglioside (GM2) mAbs |
Clone No. | AFD- E1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA, IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | Gangliosides are glycosphingolipids expressed on the cell surface membranes of most mammalian cell types. In ELISA experiments E1 showed a highly-restricted binding specificity, detecting only GM2 (not GM1 or GM3). The antibody recognises only the N-acetylneuraminic acid deratives of gangliosides; no N-glycolylneuraminic acid derivatives were recognised. |
Alternative Name | |
UniProt | |
Immunogen | The antibody was generated by injecting BALB/c mice with GM2 ganglioside incorporated into liposomes. |
Application Notes | ELISA demonstrated E1 has a highly-restricted binding specificity, binding only to GM2. IHC studies showed positivity with E1 in primary lung carcinomas, but no reactivity with corresponding normal tissues (Alfonso et al, 1995). |
Antibody First Published | Alfonso et al, 1995. T Cell-Independent Cell Response to Self- Monosialogangliosides: Primary Response Monoclonal Antibodies Hybridoma. 1995 Jun;14(3):209-16 PMID:7590782 |
Note on publication | Describes the original generation of E1 and its characterisation through ELISA and IHC. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
