Key features and details | |
Cat. No. | MABL-2554 |
Name | Anti-Osteocalcin mAbs |
Clone No. | AFD- KTM219 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody bind the C-terminal end of the human osteocalcin. |
Alternative Name | BGP; BGLAP; Bone Gla protein; Gamma-carboxyglutamic acid-containing protein |
UniProt | P02818 |
Immunogen | The original antibody was generated by repeatedly immunizing mice with C-terminal hBGP peptide (NH2-FQEAYRRFYGPVCOOH) conjugated with keyhole limpet hemocyanin. |
Application Notes | This antibody is recommended for detection of low moecular weight peptides by using open sandwich ELISA (OS-ELISA). This antibody detects C-terminal of human osteocalcin in an ELISA. The cloned VH/VL cDNA fragments of this antibody were successful in detecting C-terminal peptides as short as 6 residues in an OS-ELISA. (PMID: 17636882). The VH-MBP and VL-MBP fusion proteins of this antibody were also used in the detection of small biomarker peptide by a micro OS-ELISA integrated into a microfluidic device (PMID: 20024056). The affinity matured version of this antibody O2AG2 was also made using OS- ELISA. It is also available on request (PMID: 20123883). This antibody was also used in the development of a highly sensitive protein based open sandwich immuno-PCR for detection of small biomarkers (PMID: 24025570) |
Antibody First Published | Lim et al. Noncompetitive detection of low molecular weight peptides by open sandwich immunoassay. Anal Chem. (2007); 79(16):6193-200. PMID:17636882 |
Note on publication | Describes the generation and use of this antibody in the detection of low molecular weight peptides using open sandwich ELISA. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
