Anti-P-selectin mAbs

Cat. No.

MABL-2572

Name

Anti-P-selectin mAbs

Clone No.

AFD- PL7-6

From

Recombinant Antibody

Isotype

Engineer antibody

Application

WB, ELISA, FC

Species Reactivity

Human

Basic Information

Specificity

This antibody is specific for human   P-selectin, which is expressed by activated platelets.

Alternative Name

GMP-140; SELP; CD62 antigen-like family   member P; Granule membrane protein 140; Leukocyte-endothelial cell adhesion   molecule 3; LECAM3; Platelet activation dependent granule-external membrane   protein; PADGEM; CD62P

UniProt

P16109

Immunogen

This antibody was raised by immunising   BALB/c mice with washed platelets.

Application Notes

This antibody has been used in flow   cytometric detection of thrombin-stimulated platelets (Katayama et al, 1992),   and a chimeric mouse/human version of this antibody also preferentially   reacts with activated platelets (Kurome et al, 1994). This could enable the   detection of platelets aggregating at sites of blood vessel injury, or the   delivery of clot-dissolving drugs. The specificity of this antibody for CD62   has been confirmed in western blot and ELISA (Katayama et al, 1992), and ELISA analysis based on this antibody has been used to assess P-selectin   levels in plasma sample of patients post-angina (Ikeda et al, 1995).

Antibody First   Published

Katayama et al. A monoclonal antibody-based   enzyme immunoassay for human GMP-140/P-selectin Journal of Immunological   Methods, Volume 153, Issues 1–2, 30 August 1992, Pages 41-48 PMID:1381405

Note on publication

Describes the original generation of this   antibody, and its use in ELISA.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.