Key features and details | |
Cat. No. | MABL-2598 |
Name | Anti-pAKT mAbs |
Clone No. | AFD- AbAb-pAKT |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, WB, ELISA |
Species Reactivity | Human, Mouse |
Basic Information | |
Specificity | This antibody binds phosphorylated phosphoinositide phospholipase C-gamma, which mediates the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). It also plays an important role in the regulation of intracellular signaling cascades. |
Alternative Name | AKT1; PKB; RAC; RAC-alpha serine/threonine-protein kinase; Protein kinase B; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha |
UniProt | P31749 |
Immunogen | The original antibody was generated by immunizing rabbits with recombinant phosphorylated peptide of protein kinase B (PHFPQF(pS)YSAS) conjugated with KLH as an immunogen. |
Application Notes | The binding reactivity of this antibody to recombinant phosphorylated protein kinase B peptide was confirmed using ELISA. The binding reactivity was further confirmed using western blot, of pAkt from NIH3T3 cell lysates. The scFv version of this antibody was used fused to human IgG1 using a suitable linker for the generation of scFv-Fc mini-antibody. This minibody was capable of immunoprecipitating pAkt proteins. (US20090233358). |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
