Key features and details | |
Cat. No. | MABL-2597 |
Name | Anti-PAI-1 mAbs |
Clone No. | AFD-Nb64 (VHH-2w-64) |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | functional assay, inhibit, neutralize |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody binds human PAI-1 in the hI‐s5A loop comprising amino acid residues 300 to 314 (RQFQADFTSLSDQ). Plasminogen activator inhibitor-1 (PAI-1), is a serine protease inhibitor, a unique member of the serpin superfamily which plays an important role in fibrinolysis and is an established risk factor for cardiovascular diseases. It a primary inhibitor of tissue-type plasminogen activator (PLAT) and urokinase-type plasminogen activator (PLAU). As PLAT inhibitor, it is required for fibrinolysis down-regulation and is responsible for the controlled degradation of blood clots. As PLAU inhibitor, it is involved in the regulation of cell adhesion and spreading . |
Alternative Name | SERPINE1; PAI1; PAI; PLANH1; Plasminogen activator inhibitor 1; Endothelial plasminogen activator inhibitor; Serpin E1 |
UniProt | P05121 |
Immunogen | The original antibody was isolated from a nanobody library generated by immunization of three alpacas with three different PAI-1 variants. |
Application Notes | This antibody is capable of inhibiting both glycosylated and non-glycosylated PAI-1 variants through a substrate-inducing mechanism. The profibrinolytic effect of this antibody was confirmed using an in vitro clot lysis assay (PMID: 27604413). This antibody neutralized PAI-1 by redirecting (95-97%) of the reaction with two chain (tc) tPA and uPA plasminogen activators to the substrate branch. This antibody was less effective with single chain proenzymes (Komissarov et al, 2019). |
Antibody First Published | Zhou et al. Generation and in vitro characterization of inhibitory nanobodies towards plasminogen activator inhibitor 1. Thromb Haemost. 2016 Nov 30;116(6):1032-1040. PMID:27604413 |
Note on publication | Describes the generation of 5 inhibitory nanobodies against PAI-1. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
